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KMID : 0903520090520050539
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 5 p.539 ~ p.544
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Antioxidant Properties of Proanthocyanidin Fraction Isolated from Wild Grape (Vitis amurensis) Seed
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Lee Hye-Ryun
Bak Min-Ji
Jeong Woo-Sik
Kim Young-Chan
Chung Shin-Kyo
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Abstract
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In order to investigate the utilization of wild grape by-products, isolation of proanthocyanidin from the wild grape seed (Vitis amurensis) and its antioxidant capacities were examined. 70% acetone crude extract of the wild grape seed was fractionated. The ethyl acetate fraction was applied to Sephadex LH-20 column chromatography, which was eluted with 50%, 75% methanol, and 75% acetone, respectively, and 9 fractions were collected, finally. Their proanthocyanidin characteristics and contents were investigated by vanillin-H2SO4 and BuOH-HCl methods. The Fr. 5 has the highest proanthocyanidin content (86.00±6.74 g%) among all the fractions. Fr. 6-2 was identified as (+)- catechin by LC-MS analysis. The antioxidant activities of the proanthocyanidin were evaluated by total phenolic contents, DPPH radical scavenging, and FRAP assays. Fr. 8 shows the strongest antioxidant activities with high proanthocyanidin content. The wild grape seed containing fairly high amounts of proanthocyanidin could be utilized as a natural antioxidant material.
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KEYWORD
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antioxidant activity, proanthocyanidin, Sephadex LH-20 column chromatography, wild grape (Vitis amurensis) seed
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